Fine Mapping and Candidate-Gene Analysis of an open glume multi-pistil 3 (mp3) in Rice (Oryza sativa L.)

The rice mutant mp3 was derived from an indica–japonica cross between Rejing35 and XieqingzaoB, producing inconstant number of pistils ranging one to four in a floret at heading stage, which also developed open-glume with or two seeds twin seedlings mature seedling stage. Several altered characteristics, including filling grain panicle–1 (62.90), grain-setting rate (60.48%) yield plant–1 (13.42 g), decreased but increase 1000-grain weight (36.87 g) observed. Genetic analysis revealed that the phenotype controlled by single recessive gene. Using chromosome walking strategy F2 population 02428/mp3, gene fine mapped L3-135 RM7576, physical distance 30.617 kb on 3. Four candidate genes were found this region referred genome annotations. LOC_Os03g11614/OsMADS1 corresponded phenotype. Sequencing showed no sequence alterations coding promoter mp3. may be allelic three previously reported different floral organ phenotypes rice. Therefore, provided novel perspective biological function OsMADS1 development organ.